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Automated particle correspondence and accurate tilt-axis detection in tilted-image pairs

Authors
Journal
Journal of Structural Biology
1047-8477
Publisher
Elsevier
Volume
187
Issue
1
Identifiers
DOI: 10.1016/j.jsb.2014.03.017
Keywords
  • Particle Correspondence
  • Tilted Pairs
  • Tilt-Axis Detection
Disciplines
  • Computer Science

Abstract

Abstract Tilted electron microscope images are routinely collected for an ab initio structure reconstruction as a part of the Random Conical Tilt (RCT) or Orthogonal Tilt Reconstruction (OTR) methods, as well as for various applications using the “free-hand” procedure. These procedures all require identification of particle pairs in two corresponding images as well as accurate estimation of the tilt-axis used to rotate the electron microscope (EM) grid. Here we present a computational approach, PCT (particle correspondence from tilted pairs), based on tilt-invariant context and projection matching that addresses both problems. The method benefits from treating the two problems as a single optimization task. It automatically finds corresponding particle pairs and accurately computes tilt-axis direction even in the cases when EM grid is not perfectly planar.

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