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Nerve growth factor and neurotrophin-3 enhance neurite outgrowth and up-regulate the levels of messenger RNA for growth-associated protein GAP-43 and Tα1 α-tubulin in cultured adult rat sensory neurones

Authors
Journal
Neuroscience Letters
0304-3940
Publisher
Elsevier
Publication Date
Volume
185
Issue
1
Identifiers
DOI: 10.1016/0304-3940(94)11215-5
Keywords
  • Neurotrophin
  • Axonal Outgrowth
  • Regeneration
  • Cytoskeletal Proteins
  • Sensory Neurones
  • Culture
  • Mrna Expression
Disciplines
  • Biology

Abstract

Abstract The effect of nerve growth factor (NGF) and neurotrophin-3 (NT-3) on neurite outgrowth was related to the level of mRNA for the growth-associated protein GAP-43 and the neurone specific α -tubulin, Tα1, in dissociated cultures of adult rat sensory neurones. Treatment with NGF or NT-3 for days enhanced neurite outgrowth in a dose-dependent manner and by 7-fold and 5-fold, respectively, over control at the highest concentrations used. NGF and NT-3 elevated the level of mRNA encoding GAP-43 by 2.3-fold and Tα1 α-tubulin by 3.2–3.5-fold. The estimated ED 50 values were 0.1–0.3 ng/ml for NGF and 2 ng/ml for NT-3 for both neurite outgrowth and mRNA up-regulation.

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