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Stimulation of antiviral cellular immune responses by therapeutic vaccination of HIV-1-infected patients with dendritic cells transfected with gag, tat, rev and nef mRNA

Authors
Journal
Retrovirology
1742-4690
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Volume
8
Identifiers
DOI: 10.1186/1742-4690-8-s2-p76
Keywords
  • Poster Presentation
Disciplines
  • Biology
  • Medicine

Abstract

Stimulation of antiviral cellular immune responses by therapeutic vaccination of HIV-1-infected patients with dendritic cells transfected with gag, tat, rev and nef mRNA POSTER PRESENTATION Open Access Stimulation of antiviral cellular immune responses by therapeutic vaccination of HIV-1-infected patients with dendritic cells transfected with gag, tat, rev and nef mRNA Ellen Van Gulck1, Viggo F Van Tendeloo2,3*, Erika Vlieghe4, Marc Vekemans4, Ann Van de Velde2,3, Evelien Smits2,3, Sébastien Anguilie2,3, Nathalie Cools2,3, Barbara Stein2,3, Griet Nijs2,3, Herman Goossens2, Liesbet Mertens4, Winni De Haes1, Céline Merlin1, Derek Atkinson1, Johnsson Wong5, Eric Florence4, Guido Vanham1,6, Zwi N Berneman2,3 From Frontiers of Retrovirology 2011 Amsterdam, The Netherlands. 3-5 October 2011 Background In an attempt to raise protective antiviral immunity, dendritic cell (DC) immunotherapy was evaluated in 6 adults infected with human immunodeficiency virus (HIV)-1 and stable under antiretroviral therapy (HAART). Methods Autologous monocyte-derived DC electroporated with mRNA encoding Gag and TatRevNef fusion protein were injected 4 times at 4 weeks interval, while patients remained on HAART. Feasibility, safety and immuno- genicity were investigated. Results DC vaccine preparation and administration was success- ful in all patients and only mild adverse events such as skin reactions were seen. DC vaccination induced immune responses that have been reported to be related to control of HIV-1 replication. There was a significant increase post-as compared to pre-DC vaccination, in magnitude - in particular to Gag – and breadth of HIV- 1-specific interferon (IFN)-g response and T-cell prolif- eration. Breadth of IFN-g response and T-cell prolifera- tion were correlated with both CD4+ and CD8+ polyfunctional T-cell responses. Importantly, DC vacci- nation induced or increased the capacity of autologous CD8+ T-cells to suppress superinfection of CD4+ T- cells with the vaccine-related III

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