Affordable Access

Publisher Website

A novel method for RNA interference in neurons using enhanced green fluorescent protein (EGFP)-transgenic rats

Authors
Journal
Neuroscience Research
0168-0102
Publisher
Elsevier
Publication Date
Volume
61
Issue
2
Identifiers
DOI: 10.1016/j.neures.2008.02.008
Keywords
  • Short Interfering Rna (Sirna)
  • Immunofluorescence
  • Enhanced Green Fluorescent Protein (Egfp)
  • Growth Cone
  • Axonal Growth
  • Transgenic Mice
  • Fatty Acid-Binding Protein (Fabp)
Disciplines
  • Biology

Abstract

Abstract RNA interference (RNAi) is the simplest way of examining gene function by inhibiting expression. However, due to the low rate of introducing short interfering RNA (siRNA) into neurons, it is difficult to discriminate into which neurons that have been successfully introduced. Here, we used neurons from transgenic rats expressing enhanced green fluorescent protein (EGFP), and we simultaneously applied small interfering RNAs (siRNAs) against EGFP and a targeted gene to the EGFP-expressing neurons. EGFP fluorescence and immunoreactivity of the protein were then assessed by immunofluorescence microscopy. Quantitative analysis of the immunofluorescence confirmed that loss of EGFP closely correlates with loss of the target protein. These results indicate that this method can be used in a wider range of the neuroscientific research, especially in genome-wide studies.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Generation of transgenic rats expressing enhanced...

on The Journal of reproduction an... December 2003

Improved method to raise polyclonal antibody using...

on Acta Biochimica et Biophysica... February 2008

[Suppression of enhanced green fluorescent protein...

on Zhonghua yi xue yi chuan xue z... August 2005
More articles like this..