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Determination of nociceptin–orphanin FQ metabolites by capillary LC–MS

Authors
Journal
Brain Research Protocols
1385-299X
Publisher
Elsevier
Publication Date
Volume
6
Identifiers
DOI: 10.1016/s1385-299x(00)00035-0
Keywords
  • Nociceptin–Orphanin Fq (Ofq/N)
  • Mass Spectrometry
  • Capillary High-Performance Liquid Chromatography
  • Peptidase
  • Neuropeptide
  • Metabolism

Abstract

Abstract Nociceptin–orphanin FQ (OFQ/N) is a newly discovered peptide involved in pain transmission. The method is described to identify metabolic pathway of this neuropeptide in the spinal cord of rats using capillary size-exclusion liquid chromatography coupled to electrospray ionization mass spectrometry. The applied technique is rapid and selective, and allows for simultaneous measurement and quantitation of several fragments in the incubation mixture.

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