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Purine and pyrimidine nucleotide metabolism of vascular smooth muscle cells in culture

Authors
Journal
General Pharmacology The Vascular System
0306-3623
Publisher
Elsevier
Publication Date
Volume
27
Issue
5
Identifiers
DOI: 10.1016/0306-3623(95)02087-x
Keywords
  • Uracil
  • Uridine
  • Uric Acid
  • Cytidine
  • Vascular Tissue
  • Utp
  • Purine And Pyrimidine Release
  • Paracrine And Autocrine Functions

Abstract

Abstract 1. 1. Cultures of vascular smooth muscle cells accumulate extracellular breakdown products of purine and pyrimidine nucleotides that, over 9 hr, represent 60±7 and 78±17%, respectively, of the intracellular nucleotide content. 2. 2. The accumulation is stimulated during contracture with 20 mM KCl or 70 μM carbachol, consistent with the notion that both pyrimidine and purine nucleotides are involved in the energetics of smooth muscle contracture. 3. 3. Because the intracellular levels of pyrimidine and purine nucleotides remain constant, it appears likely that rates of synthesis match the rates of release. 4. 4. Ectonucleotidases are present that can degrade ATP, UTP, and CTP. High-energy nucleotides may be the primary products released.

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