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Cytosolic phospholipase A2, cyclo-oxygenases and arachidonate in human stomach tumours

Authors
Journal
European Journal of Cancer
0959-8049
Publisher
Elsevier
Volume
33
Issue
9
Identifiers
DOI: 10.1016/s0959-8049(97)00168-8
Keywords
  • Experimental Oncology
Disciplines
  • Biology

Abstract

Abstract Human stomach tumours usually form more prostaglandins (PGs) than their associated normal mucosa/submucosa, but the mechanisms are not fully understood. The key enzymes are cytosolic phospholipase A2 (cPLA2, Mr 85000) and the cyclo-oxygenases (COXs) which exist in constitutive (COX-1) and inducible forms (COX-2). In human stomach tumours and associated macroscopically normal tissues, we determined the fatty acid composition by gas chromatography, amounts of cPLA2, COX-1 and COX-2 by immunoblotting with specific antibodies and cPLA2 enzyme activity using a tritiated substrate. Although compared to normal mucosa there was less arachidonate in tumours (P < 0.05), the arachidonate/total fatty acid ratio was higher. Mean amounts of cPLA2 and COX-1 and cPLA2 activity were similar in tumours and normal mucosa. However, substantial amounts of COX-2 were found in the tumours but not in the mucosa, which may explain why many gastric tumours form increased amounts of PGs. © 1997 Elsevier Science Ltd.

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