Affordable Access

Publisher Website

Failure of Manganese to Protect from Shiga Toxin

Authors
Journal
PLoS ONE
1932-6203
Publisher
Public Library of Science
Publication Date
Volume
8
Issue
7
Identifiers
DOI: 10.1371/journal.pone.0069823
Keywords
  • Research Article
  • Biology
  • Microbiology
  • Bacterial Pathogens
  • Escherichia Coli
  • Microbial Pathogens
  • Model Organisms
  • Animal Models
  • Mouse
  • Toxicology
  • Toxic Agents
  • Medicine
  • Infectious Diseases
  • Bacterial Diseases
  • Hemolytic-Uremic Syndrome
Disciplines
  • Medicine

Abstract

Shiga toxin (Stx), the main virulence factor of Shiga toxin producing Escherichia coli, is a major public health threat, causing hemorrhagic colitis and hemolytic uremic syndrome. Currently, there are no approved therapeutics for these infections; however manganese has been reported to provide protection from the Stx1 variant isolated from Shigella dysenteriae (Stx1-S) both in vitro and in vivo. We investigated the efficacy of manganese protection from Stx1-S and the more potent Stx2a isoform, using experimental systems well-established for studying Stx: in vitro responses of Vero monkey kidney cells, and in vivo toxicity to CD-1 outbred mice. Manganese treatment at the reported therapeutic concentration was toxic to Vero cells in culture and to CD-1 mice. At lower manganese concentrations that were better tolerated, we observed no protection from Stx1-S or Stx2a toxicity. The ability of manganese to prevent the effects of Stx may be particular to certain cell lines, mouse strains, or may only be manifested at high, potentially toxic manganese concentrations.

There are no comments yet on this publication. Be the first to share your thoughts.