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The effect of Puerariae radix on lipoprotein metabolism in liver and intestinal cells

BioMed Central
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  • Research Article
  • Biology
  • Medicine

Abstract ral BMC Complementary and ss BioMed CentAlternative Medicine Open AcceResearch article The effect of Puerariae radix on lipoprotein metabolism in liver and intestinal cells Jeong-Sook Lee1, John Mamo2, Nerissa Ho2 and Sebely Pal*2 Address: 1Department of Food and Nutrition, Kosin University, Pusan 6660-701, Korea and 2Department of Nutrition, Dietetics and Food Sciences, Curtin University, Perth, Western Australia, Australia Email: Jeong-Sook Lee - [email protected]; John Mamo - [email protected]; Nerissa Ho - [email protected]; Sebely Pal* - [email protected] * Corresponding author Puerariae radixApoB100ApoB48HepG2 cellsCaCo2 cellscholesterol Abstract Background: Animal studies investigating the beneficial effects of Puerariae radix on cardiovascular disease have suggested this plant possesses anti-diabetic and lipid lowering properties. However, the exact mechanism by which Puerariae radix affects lipid metabolism is currently unknown. The aim of this study was to investigate the effect of the water extract of Puerariae radix on the secretion of VLDL and chylomicrons from HepG2 liver cells and CaCo2 cells, respectively, in humans. Methods: The amount of apoB100 (a protein marker for VLDL) and apoB48 (a protein marker for chylomicrons) in cells and media were quantified by Western Blotting and enhanced chemiluminescence (ECL). Total, free and esterified cholesterol concentrations were measured by gas liquid chromatography. Results: Treatment of cells with water extract of Puerariae radix significantly decreased apoB100 production and secretion from HepG2 cells up to 66% in a dose dependent manner. The intracellular total cholesterol and free cholesterol concentration in HepG2 cells also decreased with increasing concentration of the Puerariae radix. In contrast, water extract of Puerariae radix attenuated apoB48 concentrations in cells, but not apoB48 secretion from CaCo2 enterocytes. Conclusions: Collectively, our findings suggest tha

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