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Long-chain phenols:XXI. quantitative analysis of the phenolic lipids in technical cashew nut-shell liquid, from anacardium occidentale, by high-performance liquid chromatography

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
213
Issue
2
Identifiers
DOI: 10.1016/s0021-9673(00)81911-1

Abstract

Abstract The novel separation of the constituent phenols in technical cashew nut-shell liquid from the industrial processing of Anacardium occidentale has been effected by high-performance liquid chromatography. The adsorption mode on columns of 5-μm and 10-μm Partisil and the reversed-phase partition mode with 5-μm and 10-μm Spherisorb bonded with octadecylsilane have been investigated. Determination of relative molar response values for the main constituent phenols and the use of an internal standard have led to a quantitative procedure by isocratic elution under reversed-phase partition, preferably with the solvent acetonitrile-water. Gradient elution with tetrahydrofuran and acetonitrile has also enabled the polymeric material to be estimated in the various types of technical cashew nut-shell liquid examined.

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