Affordable Access

Publisher Website

A diagonal procedure for isolating sulfhydryl peptides alkylated withN-ethylmaleimide

Authors
Publisher
Elsevier Inc.
Publication Date
Volume
107
Issue
2
Identifiers
DOI: 10.1016/0003-2697(80)90396-6
Keywords
  • Protein Analyses And Reagents

Abstract

Abstract The alkylation product of N-ethylmaleimide while stable at acid pH is hydrolyzed at alkaline pH to the corresponding N-ethylsuccinamic acid. Based on this behavior, a diagonal electrophoretic procedure for separating sulfhydryl peptides alkylated with N-ethylmaleimide from the bulk of unlabeled peptides of a proteolytic digest was developed. After a first high-voltage paper electrophoresis at pH 6.5 the paper strip is exposed to an ammonia atmosphere. Hydrolysis of the N-ethylsuccinimide moiety to N-ethylsuccinamic acid generates a new negative charge allowing ready separation of the labeled from the unlabeled peptides in a second electrophoretic run.

There are no comments yet on this publication. Be the first to share your thoughts.