Affordable Access

Publisher Website

Extracellular matrix components influence DNA synthesis of rat hepatocytes in primary culture

Authors
Journal
Experimental Cell Research
0014-4827
Publisher
Elsevier
Publication Date
Volume
167
Issue
2
Identifiers
DOI: 10.1016/0014-4827(86)90186-2

Abstract

Abstract The effects of several extracellular matrix components (EMCs)—fibronectin (Fn), laminin (Ln), type I (C-I) and type IV (C-IV) collagen—on DNA synthesis in rat hepatocytes in primary culture were examined by both quantitative scintillation spectrometry and autoradiography of [ 3H]thymidine incorporation. Hepatocytes cultured on Fn showed the most active DNA synthesis initiated by epidermal growth factor (EGF) with decreasing levels of [ 3H]thymidine uptake exhibited in the cells cultured on C-IV, C-I, and Ln, respectively. The decreasing level of DNA synthesis in hepatocytes cultured on Fn, C-IV, C-I, and Ln respectively was not influenced by cell density. The number of EGF receptors of hepatocytes was also not influenced by EMCs. These data suggest that EMCs modify hepatocyte DNA synthesis by means of post-EGF-receptor mechanisms which are regulated by both growth factors and cell density.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Extracellular matrix components influence DNA synt...

on Experimental Cell Research December 1986

The control of DNA synthesis in primary cultures o...

on Journal of Cellular Physiology February 1987

Initiation of DNA synthesis in primary fetal rat h...

on Proceedings of the Society for... February 1974

Cultured rat hepatocytes adapt their cellular glyc...

on Journal of Cellular Physiology August 1994
More articles like this..