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Electron microscopic autoradiography:A method for producing uniform monolayers of silver halide crystals using centrifuge sedimentation

Authors
Journal
Journal of Ultrastructure Research
0022-5320
Publisher
Elsevier
Publication Date
Volume
10
Identifiers
DOI: 10.1016/s0022-5320(64)80010-1

Abstract

A method has been developed to produce very uniform monolayers of silver bromide crystals over ultrathin sections for electron microscopic autoradiography. Diluted nuclear emulsions are centrifuged to sediment its crystals over the sections. By changing the dilution and amount of the centrifuged emulsion, the density of the produced layer can be controlled at will. The position of the grains is secured by a collodion film. The method makes it possible to coat several grids at the same time with a very similar emulsion layer and is reproducible from experiment to experiment. Various factors that affect the grain distribution have been studied. The localization of I 125 in the thyroid has been studied to test the method.

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