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Hyperthermia causes bovine mammary epithelial cell death by a mitochondrial-induced pathway

Authors
Journal
Journal of Thermal Biology
0306-4565
Publisher
Elsevier
Publication Date
Volume
33
Issue
1
Identifiers
DOI: 10.1016/j.jtherbio.2007.06.002
Keywords
  • Mammary Epithelial Cells
  • Apoptosis
  • Necrosis
  • Mitochondria

Abstract

Abstract The effects of mild hyperthermia on bovine mammary epithelial cells exposed to 40 °C for 1 h were studied. The results showed that cell viability, ultrastructural features as well as mitochondrial function were significantly influenced by the mild heat treatment (40 °C). There was a considerate decrease in cell viability accompanied by cell loss resulting from apoptosis and necrosis followed by G 2/M arrest. Cell death followed the typical cascade, namely decrease in the ratio of Bcl-2/Bax and mitochondrial membrane potential (Δ Ψ m), mitochondrial swelling and caspase-3 activities dramatically increased; DNA was also damaged. In conclusion, hyperthermia depresses cell viability and induces bovine mammary cell apoptosis and necrosis through the mitochondrial-triggered cell death pathway.

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