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Structural and functional homology among chicken, duck, goose, turkey and pigeon interleukin-8 proteins

Authors
Journal
Veterinary Immunology and Immunopathology
0165-2427
Publisher
Elsevier
Publication Date
Volume
125
Identifiers
DOI: 10.1016/j.vetimm.2008.03.001
Keywords
  • Interleukin (Il)-8
  • Chicken Il-8
  • Avian Species
  • Chemotaxis Assay
  • Homology
  • Phylogenetic Analysis
  • Molecular Cloning
Disciplines
  • Biology

Abstract

Abstract Interleukin (IL)-8-encoding regions of five avian species were cloned, sequenced and characterized. Each IL-8-encoding region is 312 nucleotides long and encodes IL-8 which is 103 amino acids. Pairwise sequence analysis showed that sequence identities of IL-8-encoding regions ranged from 87% to 100%. The IL-8 protein identities varied from 84% to 100%. Phylogenetic analysis indicated that IL-8-encoding regions and encoded proteins of chicken, duck, goose and turkey clustered together and evolved into a distinct phylogenetic lineage from that of pigeon which evolved into a second lineage. The results from binding reactivities of antiserum against each recombinant IL-8 (rIL-8) protein to homologous or heterologous rIL-8 proteins, chemotactic activities of each rIL-8 protein or reduction levels of the chemotactic activity of rIL-8 protein which was pretreated with homologous or heterlogous antiserum have suggested that all five IL-8 proteins were functionally active, and shared structural and functional identity with each other.

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