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Exclusive leukotriene C4synthesis by purified human eosinophils induced by opsonized zymosan

Authors
Publisher
Elsevier B.V.
Publication Date
Volume
189
Issue
2
Identifiers
DOI: 10.1016/0014-5793(85)81054-1
Keywords
  • Leukotriene C4
  • Eosinophil
  • Phagocytosis
  • Opsonized Zymosan
  • Lipoxygenase
  • Asthma

Abstract

Abstract Purified human eosinophils were challenged with N-formyl-methionyl-leucyl-phenylalanine, leukotriene B 4, platelet-activating-factor, valyl-glycyl-seryl-glutamic acid, phorbol myristate acetate, zymosan, opsonized zymosan and the calcium ionophore A23187 to induce leukotriene synthesis. Reversed-phase high performance liquid chromatography analysis demonstrated the almost exclusive synthesis of leukotriene C 4 by eosinophils of 11 healthy donors after challenge with opsonized zymosan [(22±4) × 10 6 molecules LTC4/cell, mean ± SE] or the calcium ionophore A23187 [(54 ± 7) × 10 6 molecules LTC4/cell, mean ± SE]. The other agents were not capable of inducing leukotriene formation. When in addition to opsonized zymosan N-formyl-methionyl-leucyl-phenylalanine or platelet-activating factor were added a significant increase of the leukotriene C 4 synthesis by eosinophils was observed. These results suggest that eosinophils might be triggered to produce considerable amounts of the spasmogenic leukotriene C 4 in vivo by C3b- and/or IgG-mediated mechanisms e.g. phagocytosis.

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