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Selective Enrichment of Endogenous Serum Phosphopeptides with Ultrafiltration Membrane Intercept-Titanium Dioxide Chromatography Coupled with Nano Ultra-High-Pressure Liquid Chromatography-Quadrupole-Time of Flight Mass Spectrometry

Authors
Journal
Chinese Journal of Analytical Chemistry
1872-2040
Publisher
Elsevier
Publication Date
Volume
39
Issue
12
Identifiers
DOI: 10.1016/s1872-2040(10)60485-9
Keywords
  • Ultrafiltration Membrane
  • Tio2Enrichment
  • Endogenous Serum Phosphopeptides
  • Nano Ultra-High-Pressure Liquid Chromatography-Quadrupole-Flight Time Mass Spectrometry

Abstract

Abstract A novel method coupling ultrafiltration membrane with titanium dioxide (TiO 2) chromatography was developed to remove large amounts of high-molecular-weight compounds and enrich endogenous serum phosphopeptides. The enriched endogenous serum phosphopeptides were characterized by nano ultra-high-pressure liquid chromatography-quadrupole-flight time mass spectrometry (nano UPLC-Q-TOF MS). Up to 18 endogenous phosphopeptides could be successfully identified from the serum of lung cancer. The established method improved the sensitivity and efficiency of detection of serum phosphopeptides, which might be considered a milestone in tumour biomarker discovery.

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