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Soymilk: An effective and inexpensive blocking agent for immunoblotting

Authors
Journal
Analytical Biochemistry
0003-2697
Publisher
Elsevier
Publication Date
Volume
426
Issue
1
Identifiers
DOI: 10.1016/j.ab.2012.03.028
Keywords
  • Immunoblots
  • Western Blots
Disciplines
  • Biology

Abstract

Abstract Blocking efficacy of whole soymilk, nonfat soymilk, SuperBlock, and nonfat milk was evaluated by performing standard protein immunoblotting procedures on both purified protein and crude nuclear extracts from HEK 293 cells. Nonfat soymilk was found to have superior blocking efficacy compared with other blocking agents in terms of high signal-to-noise ratio with the shortest blocking times. In addition, the presence of low concentrations of the detergent Tween 20 (0.05–0.1%, v/v) in the wash buffer as well as antibody incubations significantly lessened the background compared with including only the detergent during wash steps.

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