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Sequential immunostaining (gold/silver) and complete protein staining (AuroDye) on Western blots

Authors
Journal
Journal of Immunological Methods
0022-1759
Publisher
Elsevier
Publication Date
Volume
89
Issue
1
Identifiers
DOI: 10.1016/0022-1759(86)90035-9
Keywords
  • Colloidal Gold
  • Colloidal Iron
  • Immunogold/Silver Staining
  • Protein Blotting
  • Immunoblotting
  • Electrophoresis
Disciplines
  • Biology

Abstract

Abstract A double staining method is described which combines immunodetection with sensitive staining of the complete electropherogram on the same membrane. The method is based on the use of Tween 20 as blocking agent, and uses immunogold/silver staining of specific antigens and gold staining of the overall protein pattern with AuroDye. This double staining makes possible the exact location of an immunodetected band within a complex protein pattern.

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