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MicroRNA-21 in lung cancer: overexpression in metastasis of pulmonary adenocarcinomas and squamous cell carcinomas

Authors
Journal
BMC Proceedings
1753-6561
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Keywords
  • Poster Presentation
Disciplines
  • Biology
  • Medicine

Abstract

MicroRNA-21 in lung cancer: overexpression in metastasis of pulmonary adenocarcinomas and squamous cell carcinomas POSTER PRESENTATION Open Access MicroRNA-21 in lung cancer: overexpression in metastasis of pulmonary adenocarcinomas and squamous cell carcinomas Maria R Silva1,2*, Paulo Santos2,4,5, Isabel Velada4, Catarina Gomes1, M João d’Aguiar1, Lia Teixeira1, João Dinis1, Susana Carmona4, Luisa Cortes5, Lina Carvalho1,2,3,5 From 16th International Charles Heidelberger Symposium on Cancer Research Coimbra, Portugal. 26–28 September 2010 MicroRNAs (miRNAs) are a class of naturally occurring small non-coding RNAs that target protein-coding mRNAs at the post-transcriptional level. Expression pro- filing has identified miRNA signatures in cancers that associate with diagnosis, staging, progression, prognosis, and response to treatment. MiRNAs are ideal biomar- kers in FFPE-tissue because, unlike mRNA, miRNA integrity is affected very little by formalin fixation. Pre- vious studies have shown that miR-21 overexpression correlated with poor prognosis in NSCLC patients. In this study we investigated the expression of mir-21 in primary carcinoma and metastasis and near non- tumor parenchyma. FFPE samples from surgical specimens and biopsies of 7 pulmonary adenocarcinomas and 5 squamous cell carcino- mas and respective metastasis together with normal lung tissue from the same case; these areas were separated by laser-capture microdissection prior to miRNA analysis. The expression level of miR-21 by qRT-PCR was sig- nificantly higher in tumor tissues than in adjacent nor- mal tissues (p = 0.005). The overexpression in the metastasis samples compared to adjacent normal tissue was almost satistically significant (p = 0.051). MiR-21 was overexpressed in tumor tissues relative to adjacent non-tumor tissues. We found an increase in miR-21 expression in primary carcinoma and metastasis in pulmonary adenocarcinomas when compared with miR-21 lower expression in squamous cell carcinoma. Des

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