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Isolation and some cultural conditions ofStreptomycesspecies which produce enzymes lysingAspergillus nigercell wall

Journal of Bioscience and Bioengineering
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Abstract As the result of screening microorganisms having lytic activity towards Aspergillus niger cell wall, two Streptomyces strains were isolated. One, termed J-28, was identified as Streptomyces cinereoruber and the other, termed J-13-3, was an unknown species belonging to Streptomyces. These strains inducibly produced chitinase and α-glucanase in a medium containing A. niger cell wall; specifically, strain J-28 chiefly produced chitinase and strain J-13-3 mainly α-glucanase. The best medium for chitinase production by the former was 0.5% A. niger cell wall, 0.5% yeast extract, 0.2% K 2HPO 4, 0.1% MgSO 4·7H 2O and 0.01% FeSO 4·7H 2O, while for α-glucanase production by the latter it was the same medium except for 1% instead of 0.5% A. niger cell wall, both in shaking cultures. Reaction products in chitin hydrolysis with chitinase were N, N′-diacetyl chitobiose and chitin oligomers and those of nigeran with α-glucanase were maltose and nigeran oligomers.

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