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Rapid Induction of NF-κB Binding during Liver Cell Isolation and Culture: Inhibition byL-NAMEIndicates a Role for Nitric Oxide Synthase

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
257
Issue
1
Identifiers
DOI: 10.1006/bbrc.1999.0424

Abstract

Abstract This study is the first to demonstrate activation of NF-κB binding just 10 minutes into the commonly employed hepatocyte isolation procedure. It is further reported that the anti-oxidant Trolox can prevent the induction of NF-κB during the well established hepatocyte isolation procedure but not during their subsequent culture. However both phases of NF-κB activation are inhibited by L-NAMEintimating a role for NO production, via nitric oxide synthase. These findings demonstrate that at least 2 different signal transduction pathways are operative during hepatocyte isolation and culture. Thus further studies employing Trolox and L-NAMEwill help delineate how each pathway contributes to the generalised loss of liver function commonly observed in vitro.

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