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Expression of the neural specific protein, GAP-43, dramatically lengthens the cell cycle in fibroblasts

Authors
Journal
International Journal of Developmental Neuroscience
0736-5748
Publisher
Elsevier
Publication Date
Volume
27
Issue
6
Identifiers
DOI: 10.1016/j.ijdevneu.2009.06.013
Keywords
  • Neurogenesis
  • Cell Cycle
  • Gap-43
  • Brdu
  • Nih 3T3 Cells
Disciplines
  • Biology

Abstract

Abstract It has been demonstrated that during neurogenesis in the mammalian brain, cell-cycle lengthening in neuronal progenitors may cause them to switch from proliferation to neuron-generating division. However, little is known about the cellular mechanisms involved in lengthening of the cell cycle. Growth-associated protein-43 (GAP-43) is a nervous system-specific protein whose expression in proliferating neuroblasts is related to neurogenesis. In this study, we investigated the effect of GAP-43 on cell-cycle progression in transgenic fibroblast cells. Using cumulative bromodeoxyuridine labeling, cell-cycle kinetics in GAP-43-transgenic and control NIH 3T3 cells were analyzed. Our data demonstrate that expression of GAP-43 in fibroblasts results in lengthening of the cell cycle compared to control fibroblasts. The mechanism by which GAP-43 mediated this effect appeared to involve increasing the time spent by the cells in the G 1 phase of the cell cycle.

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