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Effects of Reactive Nitrogen Scavengers on NK-Cell-Mediated Killing of K562 Cells

Authors
Journal
Journal of Biomedicine and Biotechnology
1110-7243
Publisher
Hindawi Publishing Corporation
Publication Date
Volume
2012
Identifiers
DOI: 10.1155/2012/101737
Keywords
  • Research Article

Abstract

This study explored the effects of reactive nitrogen metabolites (RNMS) on natural-killer- (NK-) cell-mediated killing of K562 cells and the influence of RNM scavengers, such as tiopronin (TIP), glutamylcysteinylglycine (GSH), and histamine dihydrochloride (DHT), on reversing the suppressing effect of RNM. We administered exogenous and endogenous RNM in the NK + K562 culture system and then added RNM scavengers. The concentrations of RNM, TNF-β and IFN-γ, and NK-cell cytotoxicity (NCC) and the percentage of living NK cells were then examined. We found that both exogenous and endogenous RNM caused the KIR to decrease (P < 0.01); however, RNM scavengers such as TIP and GSH rescued this phenomenon dose dependently. In conclusion, our data suggests that RNM scavengers such as TIP and GSH enhance the antineoplasmic activity of NK cells.

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