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Chemical analysis of an immunoenhancing water-soluble polysaccharide of an edible mushroom,Pleurotus floridablue variant

Authors
Journal
Carbohydrate Research
0008-6215
Publisher
Elsevier
Publication Date
Volume
345
Issue
18
Identifiers
DOI: 10.1016/j.carres.2010.09.032
Keywords
  • Pleurotus Floridablue Variant
  • Mushroom Polysaccharide
  • Structure
  • Nmr Spectroscopy
  • Immunoactivation

Abstract

Abstract An immunoenhancing polysaccharide isolated from the aqueous extract of the fruit bodies of the mushroom, Pleurotus florida blue variant, was found to consist of d-glucose and d-galactose in a molar ratio of nearly 5:1. On the basis of sugar analysis, methylation analysis, periodate oxidation, Smith degradation, and NMR studies ( 1H, 13C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC), the structure of the repeating unit of the polysaccharide was established as: The molecule activated macrophages, splenocytes, and thymocytes.

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