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Rapid detection of homologously integrated DNA fragments and accurate quantitation of their copy number in transgenicAspergillus oryzaeby PCR

Authors
Journal
Journal of Bioscience and Bioengineering
1389-1723
Publisher
Elsevier
Publication Date
Volume
90
Issue
5
Identifiers
DOI: 10.1016/s1389-1723(01)80046-3
Keywords
  • Methods

Abstract

Abstract A simple and rapid method for the analysis of artificially introduced DNA fragments has been developed using competitive PCR and long and accurate PCR. The locus and the copy number of the DNA fragments in each Aspergillus oryzae transformant could be detected more rapidly and accurately by this method than by the conventional Southern hybridization method.

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