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Image analysis of two-dimensional gel electrophoresis for comparative proteomics of transgenic and non-transgenic soybean seeds

Authors
Journal
Journal of Proteomics
1874-3919
Publisher
Elsevier
Publication Date
Volume
73
Issue
8
Identifiers
DOI: 10.1016/j.jprot.2010.01.009
Keywords
  • 2D Page
  • Genetically Modified Organisms
  • Soybean
  • Biomarkers
Disciplines
  • Biology

Abstract

Abstract Considering the importance of bidimensional electrophoresis and image analysis in comparative proteomics, the parameters that influence the analysis of protein expression of transgenic and non-transgenic soybean seeds were evaluated. The loaded mass of the proteins (150–500µg), the pH separation range (3–10 or 4–7), and manual/automatic image editing were evaluated. Additionally, after optimizing the conditions, histograms and matchings were obtained in order to accurately analyze the variations (90%) in protein expression. From this, 10 proteins displayed significant differences in expression, and eight of them were characterized and identified by mass spectrometry.

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