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Inhibition of glucose-induced insulin secretion by long-term preexposure of pancreatic islets to leptin.

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
415
Issue
2
Identifiers
DOI: 10.1016/s0014-5793(97)01115-0
Keywords
  • Animals
  • Glucose/Pharmacology
  • Insulin/Secretion
  • Islets Of Langerhans/Drug Effects
  • Islets Of Langerhans/Secretion
  • Leptin
  • Male
  • Proteins/Pharmacology
  • Rats
  • Rats
  • Sprague-Dawley
  • Recombinant Proteins/Pharmacology

Abstract

Here we evaluated the effect of leptin on glucose-induced insulin secretion by normal rat pancreatic islets. We show in perifusion experiments that leptin had no acute effect on the secretory activity of beta-cells. However, following preexposure to leptin a pronounced time- and dose-dependent inhibition of both first and second phases of secretion was observed. Maximum inhibition was obtained at 24 h and with 100 nM leptin. This inhibition did not involve a decrease in cellular insulin content. It was also not observed with islets from fa/fa rats. Leptin thus inhibits insulin secretion by a mechanism which requires long-term preexposure to the hormone and which may involve alteration in beta-cell gene expression.

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