Purpose To determine the prevalence and causes of amblyopia in young adult Chinese, Indian, and Malay men in Singapore. Design A population-based cross-sectional study. Methods Best-corrected visual acuities of all Singaporean men born in the years 1978 to 1983 were measured before enlistment into military service. Those with BCVA of 6/12 or worse were examined by an ophthalmologist for the presence of amblyopia and other causes. Results Of the 122,596 men examined, there were 428 with amblyopia, an overall prevalence of 0.35%. The prevalence was similar among Chinese (0.34%), Malays (0.37%), and Indians (0.41%, P = .52). Anisometropic amblyopia was the most common type of amblyopia and strabismic amblyopia was more common in Indians. There were little racial differences in the prevalence of meridional or form deprivation amblyopia. Conclusion The overall prevalence of amblyopia was low and was similar among the three racial groups in young adult Singaporean males.