An enzyme-assisted fat extraction technique has been applied to the aqueous extraction of palm kernel oil. Palm kernel meal samples were prepared and treated with different enzymes prior to extraction. The oil was then extracted using a water floatation method. Treatments with a crude enzyme with both cellulase and a hemicellulase activities, only or in combination with a protease and a pectinase, all resulted in higher palm kernel oil yield than control treatments. Optimum yield occurred when a protease, a pectinase and the cellulase/hemicellulase enzymes were combined, each at 1% concentration. Yield was influenced by the pH of the meal suspension and the meal/water ratio during the enzyme treatment. The former seemed to affect the free fatty acid values of the enzyme-assisted extracted palm kernel oil. The observations suggested that the enzyme-assisted aqueous extraction method could be used to improve the viability of the traditional palm kernel oil process.