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Direct detection of unamplified hepatoma upregulated protein RNA in urine using gold nanoparticles for bladder cancer diagnosis

Authors
Journal
Clinical Biochemistry
0009-9120
Publisher
Elsevier
Volume
47
Identifiers
DOI: 10.1016/j.clinbiochem.2013.10.022
Keywords
  • Bladder Carcinoma
  • Gold Nanoparticles
  • Hepatoma Upregulated Protein
  • Magnetic Nanoparticles
  • Squamous Cell Carcinoma
  • Transitional Cell Carcinoma
  • Urinary Biomarkers
Disciplines
  • Medicine

Abstract

Abstract Objective To develop a gold nanoparticle (AuNP) assay for direct detection of unamplified HURP RNA in urine. Design and methods HURP RNA was extracted from urine samples (50 bladder carcinoma patients, 25 benign bladder lesions, and 25 controls) and further purified using magnetic nanoparticles (MNPs), functionalized with HURP RNA-specific oligonucleotides, and then detected by RT-PCR or gold nanoparticles. Results The developed HURP RNA AuNP assay has a sensitivity and a specificity of 88.5% and 94%, respectively, and a detection limit of 2.4nmol/L. The concordance between the HURP AuNP assay with RT-PCR after RNA purification using functionalized MNPs was 97%. Conclusions The developed colorimetric HURP RNA AuNP assay is sensitive, simple, and can aid noninvasive diagnosis of bladder cancer.

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