Affordable Access

Comparative evaluation of 2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) and 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfophenyl)2H-tetrazolium, monosodium salt (WST-8) rapid colorimetric assays for antimicrobial susceptibility testing of staphylococci and ESBL-producing clinical isolates

Publication Date
  • A New Generation Of Water Soluble Tetrazolium Salts Have Recently Become Available And In This Study
  • 2-(2-Methoxy-4-Nitrophenyl)-3-(4-Nitrophenyl)-5-(2
  • 4-Disulfophenyl)-2H-Tetrazolium
  • Monosodium Salt (Wst-8)
  • With A Previously Developed 2
  • 3-Bis[2-Methyloxy-4-Nitro-5-Sulfophenyl]-2H-Tetrazolium-5-Carboxanilide(Xtt) Colorimetric Assay To D
  • The Mics Of 6 Antibiotics Were Determined For 33 Staphylococci Using Both Colorimetric Assays And Co
  • Absolute Categorical Agreement Between The Reference And Test Methods Ranged From 79% (Cefuroxime) T
  • No Minor Or Major Errors Occurred Using Either Assay With Very Major Errors Ranging From Zero (Vanco
  • Analysis Of The Distribution Of Differences In The 1092 Dilution Mic Results Revealed Overall Agreem
  • Within The Accuracy Limits Of The Standard Test ( 1 1092 Dilution)
  • Using The Xtt And Wst-8 Assays Of 98% And 88%
  • Respectively
  • Further Studies On 31 Esbl-Producing Isolates Were Performed Using The Xtt Method With Absolute Cate
  • No Errors Were Noted For Either Ofloxacin Or Meropenem With Overall Agreement Of 91%
  • The Data Suggests That Xtt Is More Reliable And Accurate Than Wst-8 For Use In A Rapid Antimicrobial
  • (C) 2007 Elsevier B
  • V
  • All Rights Reserved


1 QUEEN’S UNIVERSITY, BELFAST RESEARCH PORTAL POLICIES ON RESEARCH PUBLICATIONS PURPOSE The purpose of this document is to outline the policies that will govern the running of the Queen’s University of Belfast (hereafter referred to as Queen’s) Research Portal. DEFINITIONS Term Definition CTA Copyright Transfer Agreement. DOI Digital Object Identifier. ISBN International Standard Book Number. Metadata The bibliographical information that describes items. PDF Portable Document Format document REF Research Excellence Framework Research Portal Is the branding given to the Queen’s Institutional Repository. SHERPA/RoMEO RoMEO is a searchable database of publisher's policies regarding the self- archiving of journal articles on the web and in Open Access repositories. Work Work is the research output of Queen’s authors which will include peer-reviewed journal articles or book chapters, in an electronic format, that is deposited in the Research Portal including the abstract, text, images and any related data. 1. POSITION OF QUEEN’S UNIVERSITY, BELFAST 1.1. The University’s position on the deposit of works in the Queen’s Research Portal and allowing for open access to these works is as follows: 1.1.1. Queen’s authors enter the bibliographic details of all their published works into Pure and access to this database is restricted to Queen’s users only. 1.1.2. When entering the bibliographic details into Pure it is possible for the Queen’s author to download publication data from online sources such as Web of Science, PubMed, arXiv and Scopus, if applicable. 1.1.3. When entering the bibliographic details into Pure Queen’s authors should deposit the full text of their substantive works 1 into the Queen’s Research Portal so that they can be made available online. 1.1.4. When a Queen’s author inputs the bibliographic details of a published work they will be alerted if the details have alread

There are no comments yet on this publication. Be the first to share your thoughts.


Seen <100 times