Affordable Access

Publisher Website

Very high-pressure capillary liquid chromatography assisted by voltage

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
1106
Identifiers
DOI: 10.1016/j.chroma.2005.05.053
Keywords
  • Liquid Chromatography
  • Voltage-Assisted Capillary Liquid Chromatography
  • Capillary Electrochromatography
  • Pressure Assisted Capillary Electrochromatography

Abstract

Abstract Capillary liquid chromatography at moderately high pressures and capillary electrochromatography (CEC) have been combined to drive the mobile phase through capillary columns packed with small diameter particles. In a column packed with 1.5 μm nonporous particles, linear velocities near 3 mm/s were observed when combining inlet pressures of 690 bar (10,000 psi) and an applied voltage of 25 kV. Optimum linear velocity for the column was achieved using a pressure-voltage combination of 350 bar (5000 psi) and 5 kV. Separation efficiencies at near optimum linear velocity agreed with those predicted by the van Deemter equation for liquid chromatography. Retention factors were observed to decrease under pressure-voltage combination as the voltage was increased; such a behavior has been attributed to Joule heating effects.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Very high-pressure capillary liquid chromatography...

on Journal of Chromatography A Feb 17, 2006

Comparison of a three-peptide separation by capill...

on Journal of Chromatography A Jan 01, 2004

Comparison of a three-peptide separation by capill...

on Journal of Chromatography A Jul 30, 2004

Performance of new prototype packed columns for ve...

on Journal of Chromatography A Jan 01, 2010
More articles like this..