Affordable Access

Publisher Website

Determination of alkaline phosphatase isoenzymes

Authors
Journal
Clinica Chimica Acta
0009-8981
Publisher
Elsevier
Publication Date
Volume
36
Issue
2
Identifiers
DOI: 10.1016/0009-8981(72)90013-7
Disciplines
  • Biology

Abstract

Abstract A method for the quantitative determination of alkaline phosphatase isoenzymes on cellusose acetate has been developed. Electrophoresis is performed utilizing barbital buffer and the substrate, 2-hydroxy-3-amido- N-(2', 4'-dimethylphenyl) -naphthalene phosphate is converted to a highly fluorescent product which is measured with a fluorometer. Subsequent staining of the same cellulose acetate strips for protein permits the precise localization of the isoenzyme forms. The results obtained show good reproducibility and the simplicity of the procedure permits screening of large numbers of specimens.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

[Determination of serum alkaline phosphatase and i...

on Nihon rinsho. Japanese journal... 1976

Determination of alkaline phosphatase isoenzymes.

on Clinica Chimica Acta February 1972

[Method for the determination of isoenzymes of alk...

on Zeitschrift für medizinische... 1974

Determination of isoenzymes of serum alkaline phos...

on Bulletin of the Calcutta Schoo... July 1971
More articles like this..