Affordable Access

Role of chemotactic factor inactivation in neutrophil chemotaxis

Journal of Laboratory and Clinical Medicine
Publication Date
  • Biology


Abstract CFIs have been implicated in the regulation of several inflammatory mediators; consequently, we have evaluated the effects of CFI on neutrophil chemotactic and lysosomal enzyme release responses to C 5-derived chemotaxins. Chemotaxis was measured by directed migration under agarose, LER by glucosaminidase release from cytochalasin B-treated neutrophils, and CFI activity by its inhibition of LER. After inactivation by CFI, C 5-fr lost their ability to stimulate neutrophils, and acquired a new chemotactic inhibitory activity. On gel chromatography, the stimulatory activity of C 5-fr and the inhibitory activity of inactivated C 5-fr eluted as separate peaks with different molecular weights. Effects of CFI on neutrophil responses to C 5-fr, ZAS, and ZAP were adverse and dose-dependent: maximal neutrophil response to C 5-fr decreased in amplitude as CFI levels increased, and a close reciprocal relationship was demonstrated between the endogenous CFI and the chemotactic activities of ZAS (r= −0.833) and ZAP (r= −0.932) in 10 healthy adults. The data suggest that CFI is a potent regulator of neutrophil response to C 5-derived inflammatory mediators.

There are no comments yet on this publication. Be the first to share your thoughts.


Seen <100 times

More articles like this

Role of chemotactic factor inactivation in neutrop...

on Journal of Laboratory and Clin... August 1981

Chemotactic cytokines: the role of leukocytic pyro...

on The Journal of Immunology February 1984

Chemotactic factors trigger their own oxidative in...

on The Journal of Immunology December 1982

Neutrophil leucocyte chemotaxis: a simplified assa...

on Journal of Immunological Metho... Aug 02, 1985
More articles like this..