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High-performance liquid chromatography coupled with negative ion tandem mass spectrometry for determination of pravastatin in human plasma

Authors
Journal
Journal of Chromatography B
1570-0232
Publisher
Elsevier
Publication Date
Volume
783
Issue
1
Identifiers
DOI: 10.1016/s1570-0232(02)00526-3
Keywords
  • Pravastatin
Disciplines
  • Biology

Abstract

Abstract A new method, using high-performance liquid chromatography/ion electrospray (negative ion) mass spectrometry, has been developed for the determination of a hydrophilic liver-specific inhibitor of the enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase, pravastatin in human plasma. In this method, plasma samples were prepared by a solid-phase extraction on C 18 Bond Elut cartridge. Chromatography was carried out with a Zorbax C 8 column. Simple isocratic chromatography conditions were used. The method has been validated in a linear range of 0.25–300 ng/ml with a coefficient of variation of 0.6–3.4%. The overall recovery was 90.5% for pravastatin and 90.8% for the internal standard β-hydroxy-lovastatin. The method is simple and reliable with a total run time of less than 2 min.

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