Affordable Access

Publisher Website

Essential Bacillus subtilis genes.

Authors
Journal
Proceedings of the National Academy of Sciences
0027-8424
Publisher
Proceedings of the National Academy of Sciences
Publication Date
Volume
100
Issue
8
Identifiers
DOI: 10.1073/pnas.0730515100
Keywords
  • Bacillus Subtilis/Cytology
  • Bacillus Subtilis/Genetics
  • Cell Division/Genetics
  • Cell Membrane/Genetics
  • Coenzymes/Genetics
  • Coenzymes/Metabolism
  • Energy Metabolism/Genetics
  • Genes
  • Bacterial
  • Genome
  • Bacterial
  • Mutation
  • Nucleotides/Genetics
  • Nucleotides/Metabolism
  • Phylogeny

Abstract

To estimate the minimal gene set required to sustain bacterial life in nutritious conditions, we carried out a systematic inactivation of Bacillus subtilis genes. Among approximately 4,100 genes of the organism, only 192 were shown to be indispensable by this or previous work. Another 79 genes were predicted to be essential. The vast majority of essential genes were categorized in relatively few domains of cell metabolism, with about half involved in information processing, one-fifth involved in the synthesis of cell envelope and the determination of cell shape and division, and one-tenth related to cell energetics. Only 4% of essential genes encode unknown functions. Most essential genes are present throughout a wide range of Bacteria, and almost 70% can also be found in Archaea and Eucarya. However, essential genes related to cell envelope, shape, division, and respiration tend to be lost from bacteria with small genomes. Unexpectedly, most genes involved in the Embden-Meyerhof-Parnas pathway are essential. Identification of unknown and unexpected essential genes opens research avenues to better understanding of processes that sustain bacterial life.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Essential Bacillus subtilis genes.

on Proceedings of the National Ac... Apr 15, 2003
More articles like this..