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Changes caused by air-drying and alcohol dehydration of spread insect sperm and fungal spore chromosome fibres

Authors
Journal
Experimental Cell Research
0014-4827
Publisher
Elsevier
Publication Date
Volume
79
Issue
1
Identifiers
DOI: 10.1016/0014-4827(73)90510-7

Abstract

Abstract The morphology and width of chromatin fibres from Schizophyllum commune basidiospore and Drosophila melanogaster sperm were studied using a modified whole-mount technique. As revealed by electron micrographs, the native chromatin fibre organization and width, about 130 Å in sperm and 30 Å in spore, were preserved if samples were stained immediately after spreading and drying the material without alcohol dehydration. Following air-drying and alcohol dehydration, visible changes of fibre morphology took place: air-drying before staining produced unspecific side-by-side aggregates of fibres, and a coarse fibrillar network was formed as a consequence of alcohol dehydration.

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