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β-Galactosidase Reporter System in Mycobacteria and Its Application in Rapid Antimycobacterial Drug Screening

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
235
Issue
3
Identifiers
DOI: 10.1006/bbrc.1997.6837

Abstract

Abstract Pathogenic mycobacteria are generally slow growing organisms and it takes several weeks to evaluate inhibitors of growth. Therefore, for rapid screening of the inhibitors of mycobacterial growth, a β-galactosidase reporter system has been described which utilises a recombinant Mycobacterium smegmatismc 2155 expressing E. coli lacZgene as the test organism. The assay is based on production of β-galactosidase in presence of drugs during growth. A correlation between β-galactosidase production and colony forming ability of mycobacteria was obtained. β-galactosidase production was inhibited within 6 h by front line standard antimycobacterial drugs like streptomycin, rifampicin, isoniazid, ethambutol, pyrazinamide and ofloxacin at their reported MICs. The assay was performed on mycobacterial cells permeabilized with chloroform and sodium dodecyl sulfate.

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