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Profile of acute immune response in Chinese amphioxus uponStaphylococcus aureusandVibrio parahaemolyticusinfection

Authors
Journal
Developmental & Comparative Immunology
0145-305X
Publisher
Elsevier
Publication Date
Volume
31
Issue
10
Identifiers
DOI: 10.1016/j.dci.2007.01.003
Keywords
  • Amphioxus
  • Invertebrate
  • Suppression Subtractive Hybridization
  • Innate Immunity
  • Microbial Challenge
  • Expression Sequence Tag
  • Gene Transcription
  • Comparative Immunology
Disciplines
  • Biology

Abstract

Abstract Amphioxus is traditionally considered as the living invertebrate most closely related to vertebrate. However, no systematic study was performed about how the amphioxus defends against the microbial invasion. Here we reported a profile of gene transcription after Staphylococcus aureus ( S.c) and Vibrio parahaemolyticus ( V.p) challenged by suppression subtractive hybridization (SSH). When compared with mammals, amphioxus has the same acute immune defense genes (lectins, metalloproteinase, lysozymes and antimicrobial peptide, etc.) as well as a similar pattern and level of temporal gene expression. In contrast, amphioxus was demonstrated to have some novel acute immune response genes in response to the microbial challenge, such as apextrin and dermatopontin, which have a 3500-fold and 900-fold induction after the V.p infection, respectively, suggesting new functions in early immune system for these two genes. Our results reported for the first time a profile of primitive immune system defense against infection in protochordate.

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