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Molecular Characterization of the vanD Gene Cluster and a Novel Insertion Element in a Vancomycin-Resistant Enterococcus Isolated in Canada

Authors
Publisher
American Society for Microbiology
Publication Date
Source
PMC
Keywords
  • Bacteriology
Disciplines
  • Biology

Abstract

A single vanD-containing Enterococcus faecium strain (N97-330) was isolated in Canada. The vanD-containing region was cloned and sequenced. Although the proteins have more than 96% identity to a previously described vanD region in BM4339, the vanSD gene contains a frameshift mutation that leads to a predicted truncated protein. Furthermore, sequence analysis of the ddl gene revealed the presence of an IS982-like element (ISEfm1) which interrupted the d-Ala–d-Ala ligase. This suggested the constitutive expression of the vanD operon, which was confirmed. Pulsed-field gel electrophoresis fingerprinting demonstrated that BM4339 was not related to N97-330 (>15 band differences). Both strains contained multiple copies of the IS982-like element.

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