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Procedure for rapid and sensitive detection of rhodanese separated by polyacrylamide gel electrophoresis

Authors
Journal
Analytical Biochemistry
0003-2697
Publisher
Elsevier
Publication Date
Volume
43
Issue
1
Identifiers
DOI: 10.1016/0003-2697(71)90105-9
Disciplines
  • Biology

Abstract

Abstract A simple method for the rapid and precise detection of rhodanese in polyacrylamide gel after electrophoresis was developed. Opaque white enzyme bands in the clear gel could be obtained with a sensitivity as low as 1 × 10 −9 unit in one or two minutes simply by immersing the gel in a suitable incubation mixture.

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