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Quantification of ricin toxin using a highly sensitive avidin/biotin enzyme-linked immunosorbent assay

Authors
Journal
Journal of the Forensic Science Society
0015-7368
Publisher
Elsevier
Publication Date
Volume
28
Issue
4
Identifiers
DOI: 10.1016/s0015-7368(88)72840-6
Keywords
  • Plant Toxins
  • Ricin
  • Elisa
  • Avidin Biotin Complex
Disciplines
  • Biology
  • Criminology

Abstract

Abstract A more sensitive quantitative immunoassay system was required for the forensic determination of ricin in animal tissue samples. A competitive enzyme-linked immunosorbent assay (ELISA) system has been previously described, with a maximum detection sensitivity of the order of Ing per 50 μl of tissue extract. This study reports the development of a highly sensitive ELISA, amplified using the avidin/biotin immunoperoxidase complex (ABC). With this system, the lower limit of detection for ricin has been improved considerably to the order of 20 pg per 100 μl of tissue extract. The method relies upon obtaining a solution of toxin by extracting tissue samples; the efficiency of this process is therefore critical for the success of the method. Extraction conditions have been examined and improved to maximise the opportunity for quantification of toxin.

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