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Growth-inhibitory glycopeptides obtained from the cell surface of cultured chick embryo fibroblasts

Authors
Journal
Experimental Cell Research
0014-4827
Publisher
Elsevier
Publication Date
Volume
154
Issue
1
Identifiers
DOI: 10.1016/0014-4827(84)90675-x

Abstract

Abstract Cell surface glycopeptides were obtained from cultured chick embryo fibroblasts (CEF) by digestion with Pronase E, and a fraction exerting growth-inhibitory activity on CEF was isolated by high performance gel permeation chromatography. The active fraction, tentatively termed cell surface glycopeptide-2 (CSGP-2), was soluble in 5% trichloroacetic acid (TCA) or 75% ethanol. It inhibited the growth of CEF reversibly at 10–20 μg sugar/ml, but did not inhibit BALB/c mouse 3T3, SV40-transformed 3T3, and human diploid cells at similar concentration. The growth-inhibitory activity of CSGP-2 was reduced or lost after digestion with neuraminidase or oxidation with sodium metaperiodate. Cellulose acetate electrophoresis revealed that CSGP-2 was a mixture of sialoglycopeptides. A similar growth inhibitor was also isolated from chicken embryonic tissues.

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