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Proposed revisions to the nomenclature for Brachyspira membrane proteins and lipoproteins

Society for General Microbiology
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  • Biology
  • Chemistry
  • Medicine


JOURNAL OF CLINICAL MICROBIOLOGY, Feb. 2008, p. 593–600 Vol. 46, No. 2 0095-1137/08/$08.00�0 doi:10.1128/JCM.01829-07 Copyright © 2008, American Society for Microbiology. All Rights Reserved. Identification of Brachyspira hyodysenteriae and Other Pathogenic Brachyspira Species in Chickens from Laying Flocks with Diarrhea or Reduced Production or Both� Anneke Feberwee,1* David J. Hampson,2 Nyree D. Phillips,2 Tom La,2 Harold M. J. F. van der Heijden,1 Gerard J. Wellenberg,1 R. Marius Dwars,3 and Wil J. M. Landman1 Animal Health Service (GD), P.O. Box 9, 7400 AA Deventer, The Netherlands1; School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia2; and Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands3 Received 14 September 2007/Returned for modification 20 November 2007/Accepted 28 November 2007 Cecal samples from laying chickens from 25 farms with a history of decreased egg production, diarrhea, and/or increased feed conversion ratios were examined for anaerobic intestinal spirochetes of the genus Brachyspira. Seventy-three samples positive in an immunofluorescence assay for Brachyspira species were further examined using selective anaerobic culture, followed by phenotypic analysis, species-specific PCRs (for Brachyspira hyodysenteriae, B. intermedia, and B. pilosicoli), and a Brachyspira genus-specific PCR with sequenc- ing of the partial 16S rRNA gene products. Brachyspira cultures were obtained from all samples. Less than half of the isolates could be identified to the species level on the basis of their biochemical phenotypes, while all but four isolates (5.2%) were speciated by using PCR and sequencing of DNA extracted from the bacteria. Different Brachyspira spp. were found within a single flock and also in cultures from single chickens, emphasizing the need to obtain multiple samples when investigating outbreaks of avian intestinal spirochetosis. The m

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