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The novel angiogenic cytokine secretoneurin promotes angiogenesis, arteriogenesis and vasculogenesis in the mouse hind-limb ischemia model

Authors
Journal
BMC Pharmacology
1471-2210
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Volume
8
Identifiers
DOI: 10.1186/1471-2210-8-s1-a38
Keywords
  • Meeting Abstract
Disciplines
  • Biology
  • Chemistry
  • Medicine

Abstract

1471-2210-8-S1-A38.fm BioMed Central Page 1 of 1 (page number not for citation purposes) BMC Pharmacology Open AccessMeeting abstract The novel angiogenic cytokine secretoneurin promotes angiogenesis, arteriogenesis and vasculogenesis in the mouse hind-limb ischemia model Wilfried Schgoer1, Margot Egger1, Arno Beer1, Markus Theurl1, Johannes Jeschke2, Ivan Tancevski1, Philipp Eller1, Andreas Ritsch1, Hildegunde Piza-Katzer2, Josef R Patsch1, Peter Schratzberger1, Reiner Fischer-Colbrie3 and Rudolf Kirchmair*1 Address: 1Department of Internal Medicine, Medical University of Innsbruck, 6020 Innsbruck, Austria, 2Department of Plastic Surgery, Medical University of Innsbruck, 6020 Innsbruck, Austria and 3Department of Pharmacology, Medical University of Innsbruck, 6020 Innsbruck, Austria Email: Rudolf Kirchmair* - [email protected] * Corresponding author Introduction Secretoneurin (SN) represents a sensory, inflammatory neuropeptide which was recently demonstrated to act as an angiogenic and vasculogenic cytokine in vitro and in vivo. The present study was conducted to test the hypoth- esis that SN may be implicated in reparative angiogenesis. Furthermore, we challenged the healing potential of SN applied as a newly generated SN gene therapy vector in the setting of limb ischemia. Methods and results We cloned the human SN coding sequence into the pAAV plasmid containing a cytomegalovirus enhancer/pro- moter sequence. To establish the bioactivity of the con- structed SN plasmid (p-SN), we transfected p-SN into COS cells and verified protein expression by SN-specific RIA. Bioactivity of recombinant SN was shown by prolif- erative and chemotactic activity on endothelial cells in vitro. Unilateral limb ischemia was induced in C57/bl6 mice by femoral artery resection. By real time PCR, west- ern blotting, SN-specific RIA and immunhistochemistry, we documented that SN is up-regulated in ischemic mus- cles. We tested whether SN gene therapy may exert cura- tive ef

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