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Spectrophotometric determination of phytic acid in plant extracts using a multi-pumping flow system

Authors
Journal
Analytica Chimica Acta
0003-2670
Publisher
Elsevier
Publication Date
Volume
474
Identifiers
DOI: 10.1016/s0003-2670(02)01008-5
Keywords
  • Phytic Acid
  • Multi-Pumping
  • Flow Analysis
  • Spectrophotometry
  • Food Analysis

Abstract

Abstract A flow-based spectrophotometric determination of phytic acid (PA) in foodstuffs involving PA extraction and reaction with Fe(III)-salicylate is proposed. Extraction is accomplished in a parallel array of ion-exchange mini-columns and the extracts are introduced into an improved flow system with multi-pumping. About 150 samples are run per hour, meaning 1.3×10 −2 μg FeCl 3·6H 2O and 7.7×10 −3 μg sodium salicylate per determination. Baseline drift is usually <0.01 absorbance per hour and the analytical signals for 5–100 mg l −1 PA range from 0.45 to 0.04 absorbance. Linearity of the analytical curve is fair ( r>0.999, n=5) and detection limit was estimated as 1.0 mg l −1 PA. Results are precise (R.S.D.<1%) and in agreement with HPLC.

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