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Tris(hydroxmethyl)aminomethane permits the expression of insulin-induced receptor loss in isolated rat adipocytes

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
102
Issue
2
Identifiers
DOI: 10.1016/s0006-291x(81)80181-7

Abstract

Summary The effect of Tris(hydroxymethyl)aminomethane on insulin-induced loss of cell-surface insulin receptors was studied in vitro using isolated rat adipocytes. In the presence of insulin, Tris(5–50mM) resulted in a dose-dependent loss of insulin receptors, whereas Tris alone was without effect. Loss of receptors after exposure to insulin and Tris was prevented by inhibiting endocytosis with 2,4,dinitrophenol or low temperature (16°), indicating Tris acts at an intracellular site distal to insulin binding. These results combined with our previous findings indicate that Tris mediates insulin-induced receptor loss by preventing insulin receptors from recycling.

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