Affordable Access

Directed evolution of a genetic circuit

Publication Date
  • Caltech Library Services
  • Biology
  • Chemistry
  • Design
  • Engineering
  • Medicine


The construction of artificial networks of transcriptional control elements in living cells represents a new frontier for biological engineering. However, biological circuit engineers will have to confront their inability to predict the precise behavior of even the most simple synthetic networks, a serious shortcoming and challenge for the design and construction of more sophisticated genetic circuitry in the future. We propose a combined rational and evolutionary design strategy for constructing genetic regulatory circuits, an approach that allows the engineer to fine-tune the biochemical parameters of the networks experimentally in vivo. By applying directed evolution to genes comprising a simple genetic circuit, we demonstrate that a nonfunctional circuit containing improperly matched components can evolve rapidly into a functional one. In the process, we generated a library of genetic devices with a range of behaviors that can be used to construct more complex circuits.

There are no comments yet on this publication. Be the first to share your thoughts.


Seen <100 times

More articles like this

Directed evolution of a genetic circuit.

on Proceedings of the National Ac... Dec 24, 2002

Directed evolution of genetic parts and circuits b...

on Nature Biotechnology January 2014
More articles like this..