Affordable Access

Publisher Website

The oocyte-specific transcription factor, Nobox, regulates the expression ofPad6, a peptidylarginine deiminase in the oocyte

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
584
Issue
16
Identifiers
DOI: 10.1016/j.febslet.2010.07.037
Keywords
  • Nobox
  • Folliculogenesis
  • Pad6
  • Transcription

Abstract

Abstract Nobox is an oocyte-specific transcriptional regulator. Nobox deficiency disrupts early folliculogenesis and the expression of oocyte-specific genes in mice. In the present study, we found that peptidylarginine deiminase 6 ( Pad6) was downregulated in Nobox-null ovaries. Pad6 is preferentially expressed in oocytes and its transcript is detectable at embryonic day 16.5. In addition, we identified one Nobox DNA-binding element (NBE) within the mouse Pad6 promoter. The NBE includes a core sequence TAATTA. Sequence-specific binding of Nobox to the TAATTA motif was confirmed. Nobox overexpression augmented transcriptional activity of a luciferase reporter driven by mouse Pad6. Our findings indicate that Nobox is a critical regulator that orchestrates oocyte-specific genes such as Pad6 during folliculogenesis.

There are no comments yet on this publication. Be the first to share your thoughts.